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Tau DGAE (297-391) 模擬AD前體原纖維產(chǎn)品介紹及相關(guān)文獻(xiàn)

更新時間：2025-08-26   點擊次數(shù)：61次

重組人 Tau dGAE (297-391) 模擬AD前體原纖維 (無肝素輔助聚集) Catalog No. SPR-502。如需購買Stressmarq公司產(chǎn)品，請聯(lián)系Stressmarq全國總代理欣博盛生物。

 

 

產(chǎn)品詳情：

Product NameTau dGAE (297-391) 模擬AD前體原纖維
Description

重組人 Tau dGAE (297-391) 模擬AD前體原纖維 (無肝素輔助聚集)

ApplicationsWB, SDS PAGE, In vitro Assay
Concentration2 mg/ml 或 5 mg/ml
Conjugates無標(biāo)簽
 
Nature重組的
Species人
Expression System大腸桿菌 (E. coli)
Amino Acid SequenceIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAE
Purity>95%
Protein Length野生型tau全長蛋白 2N4R 的片段 (297 - 391aa)

 

Properties：

Storage Buffer10mM PB pH 7.4, 10mM DTT, 200mM MgCl2
Storage Temperature-80oC
Shipping Temperature干冰. 運(yùn)輸: 該產(chǎn)品會與其他同訂單產(chǎn)品分開發(fā)貨.
Purification離子交換層析純化
Protein Size10.165 kDa
Cite This ProductHuman Recombinant Tau dGAE (297-391) AD-mimic Pre-formed Fibrils (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-502)

Certificate Of Analysis

經(jīng)SDS-PAGE & Nanodrop分析驗證蛋白純度 >95%.
Other Relevant Information為達(dá)到最佳效果請在使用前超聲處理. 請參考上神經(jīng)退行性疾病相關(guān)蛋白操作指南或產(chǎn)品說明書. 單體來源是目錄號# SPR-316.

 

Biological Description：

Alternative NamesTau aggregate, Tau PFFs, Tau PFF, Tau protein aggregate, Tau protein, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Truncated Tau Protein Aggregate, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, dGAE Tau Protein, Tau dGAEResearch AreasMT 相關(guān)蛋白, 神經(jīng)元標(biāo)記物, 神經(jīng)纖維纏結(jié)&Tau, 神經(jīng)退行性疾病, 細(xì)胞標(biāo)記物, 軸突標(biāo)記物, 微管, 神經(jīng)生物學(xué), 阿爾茨海默病, 細(xì)胞信號傳導(dǎo), 細(xì)胞骨架Swiss ProtP10636-8Scientific BackgroundFilamentous tau inclusions are a hallmark of many neurodegenerative diseases, including Alzheimer’s disease (AD) and Chronic Traumatic Encephalopathy (CTE), collectively called tauopathies. Advances in Cryo-EM have revealed that tau filaments isolated from individuals with a particular neurodegenerative disease share a distinct tau fold – i.e. an AD-isolated Tau filaments’ fold is distinct from a CTE-isolated Tau filaments’ fold (1-3). Utilizing Tau filaments with the correct disease-specific fold is an important goal towards better mimicking specific human diseases in cellular and in vivo models. Recent Cryo-EM studies have demonstrated that recombinantly generated Tau dGAE monomers will form the disease-isolated AD or CTE Tau filament folds under highly specific conditions in vitro (4, 5). StressMarq’s catalog# SPR-502 Tau dGAE (297-391) AD-mimic PFFs are purified and fibrilized under these exact published conditions that replicate the disease-isolated AD-fold (200 rpm at 37oC in 10 mM PB 10 mM DTT pH 7.4 200 mM MgCl2 for 48 hours).References1. Goedert, Eisenberg and Crowther. 2017. Propagation of Tau Aggregates and Neurodegeneration. Annu Rev Neurosci. 
2. Fitzpatrick et al. 2017. Cryo-EM structures of tau filaments from Alzheimer’s disease. Nature. 
3. Falcon et al. 2019. Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules. Nature. 
4. Lovestam et al. 2022. Assembly of Recombinant Tau into Filaments Identical to those of Alzheimer’s disease and Chronic Traumatic Encephalopathy. eLife. 
5. Lovestam et al. 2023. Disease-specific Tau Filaments Assemble via Polymorphic Intermediates. bioRxiv. 

 

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